2310555 GENE ENG LAB

Course Number 2310555
Course Credits2 (0-6-2)
Course AbbrviationGENE ENG LAB
Course Title (TH)ปฏิบัติการพัธุวิศวกรรม
Course Title (EN)Genetic Engineering Laboratory
Responsible UnitFaculty of Science, Department of Biochemistry
Type of CourseInternational Course
SemesterIntl 2nd semester
Academic Year2024
Course Coordinator
Measurement Method
Type of CourseSemester Course
Course ConditionCOREQ 2310454 or 2310554 or Consent of Faculty
Course Status
Instructors / staffs
Enrollment conditionsNone
Degree levelBachelor
Related curricularBachelor of Science in Biotechnology (2562)
Bachelor of Science in Biotechnology (2567)
Course description (TH)เทคนิคเกี่ยวกับรีคอมบิแนนท์ดีเอ็นเอ การสกัด การตัด และต่อดีเอ็นเอ การทรานสฟอร์มและการตรวจหายีนที่สนใจ
Course description (EN)Recombinant DNA technology; extraction, cutting and joining of DNA, transformation; screening of genes of interested genes
Curriculum mapping/CU-1.1: Behavioral Objectives Possessing well-rounded knowledge
/CU-1.2: Possessing in-depth knowledge
/CU-2.1: Being moral and ethical
/CU-2.2: Having an awareness of etiquette
/CU-3.1: Being able to think critically
/CU-3.2: Being able to think creatively
/CU-3.3: Having skills in problem solving
/CU-4.1: Having professional skills
CU-4.2: Having communication skills
CU-4.3: Having skills in information technology
/CU-4.4: Having mathematical and statistical skills
CU-4.5: Having management skills
/CU-5.1: Having an inquiring mind
/CU-5.2: Knowing how to learn
/CU-5.3: Having leadership qualities
CU-5.4: Maintaining well-being
CU-5.5: Being community-minded and possessing social responsibility
CU-5.6: Sustaining Thainess in a globalized world
subPLO1.1 Explain biotechnology knowledge in practice.
/subPLO1.2 Analyze biotechnology knowledge in practice.
subPLO1.3 Apply biotechnology knowledge in practice.
/PLO2 Employ biotechnology-related technology and scientific tools.
PLO3 Communicate effectively in English within the Biotechnology field.
/PLO4 Demonstrate behavior that aligns with ethical principles, moral values, and professional ethics.
PLO5 Demonstrate social responsibility, courage, and creativity.
Course learning outcome (CLO)1. Describe and conduct DNA extraction and PCR.
2. Describe and conduct DNA cloning.
3. Describe and conduct DNA site-directed mutagenesis.
4. Describe and conduct RNA extraction and Reverse-Transcription PCR (RT-PCR).
5. Write a report; analyze, interpret, conclude, and discuss results; present results.
#DateTimeLearning contentInstructorCLORemark
1Introduction of genetic engineering behavioral objectives• Describe and conduct DNA extraction and PCR
• Write a report; analyze, interpret, conclude, and discuss results; present results.
2Introduction of genetic engineering behavioral objectives• Describe and conduct DNA extraction and PCR
• Write a report; analyze, interpret, conclude, and discuss results; present results.
3DNA cloning• Describe and conduct DNA cloning
• Write a report; analyze, interpret, conclude, and discuss results; present results.
4DNA cloning• Describe and conduct DNA cloning
• Write a report; analyze, interpret, conclude, and discuss results; present results.
5DNA cloning• Describe and conduct DNA cloning
• Write a report; analyze, interpret, conclude, and discuss results; present results.
6DNA cloning• Describe and conduct DNA cloning
• Write a report; analyze, interpret, conclude, and discuss results; present results.
7Site-directed mutagenesis• Describe and conduct DNA site-directed mutagenesis
• Write a report; analyze, interpret, conclude, and discuss results; present results.
8Site-directed mutagenesis• Describe and conduct DNA site-directed mutagenesis
• Write a report; analyze, interpret, conclude, and discuss results; present results.
9Site-directed mutagenesis• Describe and conduct DNA site-directed mutagenesis
• Write a report; analyze, interpret, conclude, and discuss results; present results.
10RNA extraction• Describe and conduct RNAextraction and Reverse-TranscriptionPCR (RT-PCR)
• Write a report; analyze, interpret, conclude, and discuss results; present results.
11Reverse-Transcription PCR or RT-PCR• Describe and conduct RNAextraction and Reverse-TranscriptionPCR (RT-PCR)
• Write a report; analyze, interpret, conclude, and discuss results; present results.
12Reverse-Transcription PCR or RT-PCR• Describe and conduct RNAextraction and Reverse-TranscriptionPCR (RT-PCR)
• Write a report; analyze, interpret, conclude, and discuss results; present results.
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Teaching/learning media-
Communication channels / LMS
TypeChannel identifier / URLRemarks
Learning Management System (LMS)
E-mail
Assessment methodLevel of assessmentRelated CLOPercentage
GradingGrading SystemLetter Grad (A-F)
Grading methodNorm-referenced Grading (อิงกลุ่ม)
Minimum Passing Level (MPL)0
Reading list
Course evaluationCourse evaluation system
Details of improvement from previous evaluation
Course quality controlResponses to complaints / petitions from students