2303435 REGEN BIO

Course Number 2303435
Course Credits3 (2-3-4)
Course AbbrviationREGEN BIO
Course Title (TH)ชีววิทยาฟื้นฟูสภาวะเสื่อม
Responsible UnitFaculty of Science/ Biology
Type of CourseInternational Course
SemesterIntl 1st semester
Academic Year2024
Course Coordinator
Measurement Method
Type of CourseSemester Course
Course ConditionPRE 2303101 or 2303102
Course StatusRequired Course
Instructors / staffs
Enrollment conditionsNone
Degree levelBachelor
Related curricularBachelor of Science in Biotechnology (2562)
Bachelor of Science in Biotechnology (2567)
Course description (TH)ความสามารถฟื้นฟูสภาวะเสื่อมในสัตว์ การควบคุมและวิเคราะห์วัฏจักรของเซลล์ การชราภาพ การบาดเจ็บ และ การตายของเซลล์ ชีววิทยาของสเต็มเซลล์ การควบคุมการเปลี่ยนแปลงสเต็มเซลล์ไปทําหน้าที่จําเพาะและแหล่งอาศัยของสเต็มเซลล์ เนื้อเยื่อสัตว์และการกําเนิดอวัยวะ หลักของการเลี้ยงเซลล์แบบสองมิติ สามมิติและออร์แกนอยด์ การประยุกต์เวชศาสตร์ฟื้นฟูภาวะเสื่อม วิศวกรรมเนื้อเยื่อ
Course description (EN)Regenerative abilities in animals; regulation and analysis of cell cycle, cell senescence, cell injury and cell death; biology of stem cells; regulation of stem cell differentiation and stem cell niche; animal tissues and organogenesis; principles of 2D, 3D and organoid cultures; applications of regenerative medicine; tissue engineering.
Curriculum mappingCU-1.1: Behavioral Objectives Possessing well-rounded knowledge
/CU-1.2: Possessing in-depth knowledge
CU-2.1: Being moral and ethical
CU-2.2: Having an awareness of etiquette
/CU-3.1: Being able to think critically
CU-3.2: Being able to think creatively
CU-3.3: Having skills in problem solving
CU-4.1: Having professional skills
CU-4.2: Having communication skills
/CU-4.3: Having skills in information technology
CU-4.4: Having mathematical and statistical skills
CU-4.5: Having management skills
CU-5.1: Having an inquiring mind
CU-5.2: Knowing how to learn
CU-5.3: Having leadership qualities
CU-5.4: Maintaining well-being
CU-5.5: Being community-minded and possessing social responsibility
CU-5.6: Sustaining Thainess in a globalized world
subPLO1.1 Explain biotechnology knowledge in practice.
/subPLO1.2 Analyze biotechnology knowledge in practice.
subPLO1.3 Apply biotechnology knowledge in practice.
/PLO2 Employ biotechnology-related technology and scientific tools.
/PLO3 Communicate effectively in English within the Biotechnology field.
/PLO4 Demonstrate behavior that aligns with ethical principles, moral values, and professional ethics.
/PLO5 Demonstrate social responsibility, courage, and creativity.
Course learning outcome (CLO)1. Describe the processes of tissue regeneration in animals.
2. Describe the mechanisms controlling cell division, senescence and apoptosis in mammalian cells.
3. Explain properties, functions and niches of stem cells in the body.
4. Give examples of modern technological applications of stem cells in biological medicine and regenerative medicine.
5. Maintain in vitro culture of mammalian stem cells in laboratory.
#DateTimeLearning contentInstructorCLORemark
1Unit 1. Introduction
2Unit 2. Regenration in Nature
Lab 1: Orientation & Biosafety
3Unit 3. Cell Cycle, Cell Senescence, Cell Injury and Cell Death
Lab 2: Preparation of Media
4Unit 3. Cell Cycle, Cell Senescence, Cell Injury and Cell Death
Lab 3: Cell culture; whawing the cells, visualization, trypsinization and re-seed the cells
5Unit 4. Biology of Stem Cells
Lab 4: Cell counting using hemocytometer and plating the cells for the treatment
6Unit 4. Biology of Stem Cells
Lab 5: Cell viable assay; basic cell tratment and MTT assay
7Unit 5. Stem Cell Differentiation (+1 hr. Make up class)
Lab 6: Live cell staining; cell cycle analysis
8Unit 5. Stem Cell Differentiation (+1 hr. Make up class)
Lab 7: Cell culture technique; single cell survival assay (clonogenic assay)-I
9Unit 6. Animal tissues and Organogenesis
Lab 8: Cell culture technique; single cell survival assay (clonogenic assay)-II
10Unit 6. Animal tissues and Organogenesis
Lab 9: Cell Differentiation I
11Unit 7. 2D, 3D and organoid cultures
Lab 10: Cell Differentiation II
12Unit 8. Applications of regenerative medicine, tissue engineering
Lab 11: Student Presentation/Discussion
13Unit 9. Conclusion
Lab 12: Student Presentation/Discussion
Teaching/learning mediaPowerPoint media
Electronics and website media
Others (DVD and laboratory equipment)
Communication channels / LMS
TypeChannel identifier / URLRemarks
Learning Management System (LMS)Mycourseville
Assessment methodLevel of assessmentRelated CLOPercentage
Laboratory Pretest - Report25 %
Lab Discussion5 %
Class participation10 %
Mid-term examination30 %
Final examination30 %
GradingGrading SystemLetter Grad (A-F)
Grading methodCriterion-referenced Grading (อิงเกณฑ์)
Minimum Passing Level (MPL)50
Reading list
Require TextbookDavid Warburton. Cells, Tissue Engineering and Regenerative Medicine. 1st Edition. WSPC (December 15, 2014), 550 pages. ISBN-10: 9814612774
Require TextbookRobert Lanza, Anthony Atala. Essentials of Stem Cell Biology. 3rd Edition. Academic Press; 3rd edition (November 18, 2013) 674 pages. ISBN-10: 0124095038
Research ArticleFu J, Warmflash A, Lutolf MP.Stem-cell-based embryo models for fundamental research and translation. Nat Mater. (February 1, 2021) 20(2):132-144.
Research ArticleWang H, Yang Y, Liu J, Qian L. Direct cell reprogramming: approaches, mechanisms and progress. Nat Rev Mol Cell Biol. (June, 2021) 22(6):410-424.
Research ArticleYin X, Mead BE, Safaee H, Langer R, Karp JM, Levy O. Engineering Stem Cell Organoids. Cell Stem Cell. (Janurary 7, 2016) 18(1):25-38.
Research ArticleZakrzewski W, Dobrzyński M, Szymonowicz M, Rybak Z. Stem cells: past, present, and future. Stem Cell Res Ther. (February 26, 2019) 10(1):68.
Course evaluationCourse evaluation systemmyCourseVille
Details of improvement from previous evaluationAdjustment in content and teaching method and add new teaching media
Course quality controlResponses to complaints / petitions from studentsDirectly to the instructor