2303435 REGEN BIO
| Course Number | 2303435 | |
|---|---|---|
| Course Credits | 3 (2-3-4) | |
| Course Abbrviation | REGEN BIO | |
| Course Title (TH) | ชีววิทยาฟื้นฟูสภาวะเสื่อม | |
| Course Title (EN) | REGENERATIVE BIOLOGY | |
| Responsible Unit | Faculty of Science/ Biology | |
| Type of Course | International Course | |
| Semester | Intl 1st semester | |
| Academic Year | 2024 | |
| Course Coordinator | ||
| Measurement Method | ||
| Type of Course | Semester Course | |
| Course Condition | PRE 2303101 or 2303102 | |
| Course Status | Required Course | |
| Instructors / staffs | ||
| Enrollment conditions | None | |
| Degree level | Bachelor | |
| Related curricular | Bachelor of Science in Biotechnology (2562) | |
| Bachelor of Science in Biotechnology (2567) | ||
| Course description (TH) | ความสามารถฟื้นฟูสภาวะเสื่อมในสัตว์ การควบคุมและวิเคราะห์วัฏจักรของเซลล์ การชราภาพ การบาดเจ็บ และ การตายของเซลล์ ชีววิทยาของสเต็มเซลล์ การควบคุมการเปลี่ยนแปลงสเต็มเซลล์ไปทําหน้าที่จําเพาะและแหล่งอาศัยของสเต็มเซลล์ เนื้อเยื่อสัตว์และการกําเนิดอวัยวะ หลักของการเลี้ยงเซลล์แบบสองมิติ สามมิติและออร์แกนอยด์ การประยุกต์เวชศาสตร์ฟื้นฟูภาวะเสื่อม วิศวกรรมเนื้อเยื่อ | |
| Course description (EN) | Regenerative abilities in animals; regulation and analysis of cell cycle, cell senescence, cell injury and cell death; biology of stem cells; regulation of stem cell differentiation and stem cell niche; animal tissues and organogenesis; principles of 2D, 3D and organoid cultures; applications of regenerative medicine; tissue engineering. | |
| Curriculum mapping | CU-1.1: Behavioral Objectives Possessing well-rounded knowledge | |
| / | CU-1.2: Possessing in-depth knowledge | |
| CU-2.1: Being moral and ethical | ||
| CU-2.2: Having an awareness of etiquette | ||
| / | CU-3.1: Being able to think critically | |
| CU-3.2: Being able to think creatively | ||
| CU-3.3: Having skills in problem solving | ||
| CU-4.1: Having professional skills | ||
| CU-4.2: Having communication skills | ||
| / | CU-4.3: Having skills in information technology | |
| CU-4.4: Having mathematical and statistical skills | ||
| CU-4.5: Having management skills | ||
| CU-5.1: Having an inquiring mind | ||
| CU-5.2: Knowing how to learn | ||
| CU-5.3: Having leadership qualities | ||
| CU-5.4: Maintaining well-being | ||
| CU-5.5: Being community-minded and possessing social responsibility | ||
| CU-5.6: Sustaining Thainess in a globalized world | ||
| subPLO1.1 Explain biotechnology knowledge in practice. | ||
| / | subPLO1.2 Analyze biotechnology knowledge in practice. | |
| subPLO1.3 Apply biotechnology knowledge in practice. | ||
| / | PLO2 Employ biotechnology-related technology and scientific tools. | |
| / | PLO3 Communicate effectively in English within the Biotechnology field. | |
| / | PLO4 Demonstrate behavior that aligns with ethical principles, moral values, and professional ethics. | |
| / | PLO5 Demonstrate social responsibility, courage, and creativity. | |
| Course learning outcome (CLO) | 1. Describe the processes of tissue regeneration in animals. | |
| 2. Describe the mechanisms controlling cell division, senescence and apoptosis in mammalian cells. | ||
| 3. Explain properties, functions and niches of stem cells in the body. | ||
| 4. Give examples of modern technological applications of stem cells in biological medicine and regenerative medicine. | ||
| 5. Maintain in vitro culture of mammalian stem cells in laboratory. |
| # | Date | Time | Learning content | Instructor | CLO | Remark |
|---|---|---|---|---|---|---|
| 1 | Unit 1. Introduction | • | ||||
| 2 | Unit 2. Regenration in Nature | • | ||||
| Lab 1: Orientation & Biosafety | ||||||
| 3 | Unit 3. Cell Cycle, Cell Senescence, Cell Injury and Cell Death | |||||
| Lab 2: Preparation of Media | ||||||
| 4 | Unit 3. Cell Cycle, Cell Senescence, Cell Injury and Cell Death | |||||
| Lab 3: Cell culture; whawing the cells, visualization, trypsinization and re-seed the cells | ||||||
| 5 | Unit 4. Biology of Stem Cells | |||||
| Lab 4: Cell counting using hemocytometer and plating the cells for the treatment | ||||||
| 6 | Unit 4. Biology of Stem Cells | |||||
| Lab 5: Cell viable assay; basic cell tratment and MTT assay | ||||||
| 7 | Unit 5. Stem Cell Differentiation (+1 hr. Make up class) | |||||
| Lab 6: Live cell staining; cell cycle analysis | ||||||
| 8 | Unit 5. Stem Cell Differentiation (+1 hr. Make up class) | |||||
| Lab 7: Cell culture technique; single cell survival assay (clonogenic assay)-I | ||||||
| 9 | Unit 6. Animal tissues and Organogenesis | |||||
| Lab 8: Cell culture technique; single cell survival assay (clonogenic assay)-II | ||||||
| 10 | Unit 6. Animal tissues and Organogenesis | |||||
| Lab 9: Cell Differentiation I | ||||||
| 11 | Unit 7. 2D, 3D and organoid cultures | |||||
| Lab 10: Cell Differentiation II | ||||||
| 12 | Unit 8. Applications of regenerative medicine, tissue engineering | |||||
| Lab 11: Student Presentation/Discussion | ||||||
| 13 | Unit 9. Conclusion | |||||
| Lab 12: Student Presentation/Discussion | ||||||
| 14 | ||||||
| 15 | ||||||
| 16 |
| Teaching/learning media | PowerPoint media | ||
| Electronics and website media | |||
| Others (DVD and laboratory equipment) | |||
| Communication channels / LMS | |||
| Type | Channel identifier / URL | Remarks | |
| Learning Management System (LMS) | Mycourseville | ||
| Assessment method | Level of assessment | Related CLO | Percentage |
| Laboratory Pretest - Report | 25 % | ||
| Lab Discussion | 5 % | ||
| Class participation | 10 % | ||
| Mid-term examination | 30 % | ||
| Final examination | 30 % | ||
| Grading | Grading System | Letter Grad (A-F) | |
| Grading method | Criterion-referenced Grading (อิงเกณฑ์) | ||
| Minimum Passing Level (MPL) | 50 | ||
| Reading list | |||
| Type | Title | Remarks | |
| Require Textbook | David Warburton. Cells, Tissue Engineering and Regenerative Medicine. 1st Edition. WSPC (December 15, 2014), 550 pages. | ISBN-10: 9814612774 | |
| Require Textbook | Robert Lanza, Anthony Atala. Essentials of Stem Cell Biology. 3rd Edition. Academic Press; 3rd edition (November 18, 2013) 674 pages. | ISBN-10: 0124095038 | |
| Research Article | Fu J, Warmflash A, Lutolf MP.Stem-cell-based embryo models for fundamental research and translation. Nat Mater. (February 1, 2021) 20(2):132-144. | ||
| Research Article | Wang H, Yang Y, Liu J, Qian L. Direct cell reprogramming: approaches, mechanisms and progress. Nat Rev Mol Cell Biol. (June, 2021) 22(6):410-424. | ||
| Research Article | Yin X, Mead BE, Safaee H, Langer R, Karp JM, Levy O. Engineering Stem Cell Organoids. Cell Stem Cell. (Janurary 7, 2016) 18(1):25-38. | ||
| Research Article | Zakrzewski W, Dobrzyński M, Szymonowicz M, Rybak Z. Stem cells: past, present, and future. Stem Cell Res Ther. (February 26, 2019) 10(1):68. | ||
| Course evaluation | Course evaluation system | myCourseVille | |
| Details of improvement from previous evaluation | Adjustment in content and teaching method and add new teaching media | ||
| Course quality control | Responses to complaints / petitions from students | Directly to the instructor |