Course Number 2305452 
Course Credits1 (0-3-0)
Course AbbrviationPLANT TIS CUL LAB
Course Title (TH)ปฏิบัติการเลี้ยงเนื้อเยื่อพืช
Responsible UnitFaculty of Science/ Botany
Type of CourseInternational Course
SemesterIntl 2nd semester
Academic Year2024
Course Coordinator
Measurement Method
Type of CourseSemester Course
Course ConditionCOREQ 2305451
Course Status
Instructors / staffs
Enrollment conditionsNone
Degree levelBachelor
Related curricularBachelor of Science in Biotechnology (2562)
Bachelor of Science in Biotechnology (2567)
Course description (TH)การสาธิตและฝึกปฏิบัติการเทคนิคการเลี้ยงเนื้อเยื่อพืชในหลอดแก้ว
Course description (EN)Demonstrations and laboratory practices on techniques of in vitro plant tissue culture.
Curriculum mappingCU-1.1: Behavioral Objectives Possessing well-rounded knowledge
/CU-1.2: Possessing in-depth knowledge
CU-2.1: Being moral and ethical
CU-2.2: Having an awareness of etiquette
/CU-3.1: Being able to think critically
/CU-3.2: Being able to think creatively
CU-3.3: Having skills in problem solving
/CU-4.1: Having professional skills
CU-4.2: Having communication skills
CU-4.3: Having skills in information technology
CU-4.4: Having mathematical and statistical skills
CU-4.5: Having management skills
CU-5.1: Having an inquiring mind
CU-5.2: Knowing how to learn
CU-5.3: Having leadership qualities
CU-5.4: Maintaining well-being
CU-5.5: Being community-minded and possessing social responsibility
CU-5.6: Sustaining Thainess in a globalized world
/subPLO1.1 Explain biotechnology knowledge in practice.
subPLO1.2 Analyze biotechnology knowledge in practice.
subPLO1.3 Apply biotechnology knowledge in practice.
/PLO2 Employ biotechnology-related technology and scientific tools.
/PLO3 Communicate effectively in English within the Biotechnology field.
PLO4 Demonstrate behavior that aligns with ethical principles, moral values, and professional ethics.
PLO5 Demonstrate social responsibility, courage, and creativity.
Course learning outcome (CLO)1. Explain the characteristics of plant tissue culture work, the general working process, and the allocation of space in the tissue culture laboratory.
2. Prepare equipment, glassware, and media for plant tissue culture using an aseptic technique.
3. Perform methods of plant tissue culture, including preparing plant tissues, eliminating contaminants from tissues, and separating tissues for cultivation.
4. Seek knowledge and experience in the application of plant tissue culture, and plan simple experiments based on academic literature research.
#DateTimeLearning contentInstructorCLORemark
11. Introduction and set up a plant tissue culture lab
22. Medium preparation
33. Basic technique of aseptic plant tissue transfer
44. Explant disinfection technique and starting aseptic culture
55. Effect of auxin / cytokinin ratio on in vitro organogenesis
66. Proposal presentation (Using Plant Tissue Culture for Commercialization)
77. Student Project I
88. Student Project II
99. Student Project III
1010. Plant transformation
1111. Plant cell culture
1212. Exflasking of plantlets to greenhouse
1313. Student Project IV
1414. Lab Clean up and Report Preparation
1515. Lab presentation and discussion
Teaching/learning media
Communication channels / LMS
TypeChannel identifier / URLRemarks
Learning Management System (LMS)
Assessment methodLevel of assessmentRelated CLOPercentage
Class attendance and participation20 %
Lab results and performances20 %
Lab report20 %
Project report and proposal40 %
GradingGrading SystemLetter Grad (A-F)
Grading methodCriterion-referenced Grading (อิงเกณฑ์)
Minimum Passing Level (MPL)50
Reading list
Text bookDavey, M. R. and Anthony, P. 2010. Plant Cell Culture : Essential Methods. Wiley-Blackwell, Chichester, West Sussex, UK.None
Text bookGeorge, E.F., Hall, M.A. and de Klerk, G-J (eds) 2008 Plant Propagation by Tissue Culture. 3 rd ed. Springer, Dordecht, The Netherlands.None
Text bookKyte, L. and Kleyn, J. G.1996. Plants From Test Tubes: An Introduction to Micropropagation. 3rd ed., Portland, Or: Timber Press.None
Text bookSmith R. H.2012. PlantTissue Culture:Techniquesand Experiments.3 rd ed. Amsterdam: Academic Press.None
Research articlePhillips, G. C.and Garda, M..2019. Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology Plant55 (3), p242-257.None
Course evaluationCourse evaluation systemmyCourseVille
Details of improvement from previous evaluation
Course quality controlResponses to complaints / petitions from studentsDirectly to the instructor